SUMO-2/3

By post-translational covalent conjugation to other proteins within the cell, small ubiquitin-related modifiers (SUMOs) are able to regulate the cell-cycle through a common enzyme cascade involving the same E1 activating and E2 conjugating enzymes. In vertebrates, three SUMO proteins have been identified: SUMO-1, and the highly similar SUMO-2 and SUMO-3 (often referred to as SUMO-2/3). Comparably, one SUMO protein is expressed by invertebrates. Post-translational modifications by SUMO are thought to regulate multiple processes, including transcription, subcellular protein localization, and the stability of proteins.

Small ubiquitin-related modifier 2, Small ubiquitin-related modifier 3

Full length human SUMO-2

The antibody recognizes SUMO-2 and SUMO-3, but shows no detectable cross reactivity with SUMO-1.

8A2

IgG

None

Lyophilized protein G purified in PBS pH7.4

If reconstituted with deionized water in 100 µl: WB 1:1000, IF 1:2000. Optimal dilution has to be determined by the user.

Research Use Only

Lyophilized antibodies can be kept at 4ºC for up to 3 months and should be kept at -20ºC for long-term storage (2 years). To avoid freeze-thaw cycles, reconstituted antibodies should be aliquoted before freezing for long-term (1 year) storage (-80ºC) or kept at 4ºC for short-term usage (2 months). For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made with the assay buffer. After the maximum long-term storage period (2 years lyophilized or 1 year reconstituted) antibodies should be tested in your assay with a standard sample to verify if you have noticed any decrease in their efficacy. To limit antibody loss or degradation, BSA (final concentration 1%) and sodium azide (final concentration 0.02%) can be added to the suggested first dilution. It is important to first verify if those preservatives are compatible with your assay.